Ontario Provincial Police have been forced to close all of the lanes of the busy 401 Highway, for the second time in just one day. Roads have been closed between Shannonville Road and Marysville Road in Tyendinaga Township, Ontario, following a serious, five-vehicle collision on the 401 in the eastbound lanes.\nAccording to initial reports from the scene, at least five vehicles were involved in the accident on the 401 Highway, and one woman is reported to have been killed as a result of the crash. Her family are believed to be present at the scene.\nTwo Ornge helicopters were initially requested at the scene of the collision, but both were later cancelled, and land ambulances are now present.\nTraffic in the eastbound lanes is at a complete standstill, and the Ontario Provincial Police (OPP) are reporting that the road closures that are in place are expected to last between six and eight hours. Police are urging Ontario motorists to completely avoid the area if possible, or travel on the emergency detour routes.\nAlternative routes have been arranged through Marysville Road to Deseronto Road, then south back to Highway 401.\nCOLLISION: #Hwy401 EB between #Shannonville Rd and #Marysville Rd - Multiple vehicles, blocking lanes. Emergency services enroute. ^jt— OPPCommunicationsER (@OPP_COMM_ER) July 12, 2019\n#Hwy401 EB is closed for six to eight hours as @OPP_ER deal with a serious collision near the Beechwood Rd. overpass in #Napanee that happened approx. 5:30 a.m. Traffic is being rerouted on the EDR exiting at Deseronto Rd. and re-entering the highway at CR 41. @WhigStandard #ygk pic.twitter.com/MRm5WPjSrz— Meghan Balogh (@megatthewhig) July 12, 2019\nAs if this crash isn't bad enough, the nightmare on Highway 401 only gets worse, as police are also having to deal with motorists going the wrong way down the highway to avoid the terrible traffic.\nIn a tweet, the OPP East said, “If you are caught in the traffic backlog DO NOT DRIVE THE WRONG WAY ON THE HIGHWAY. Officers will work to help drivers get off the road as quickly and safely as possible.”\nIf you are caught in the traffic backlog DO NOT DRIVE THE WRONG WAY ON THE HIGHWAY. Officers will work to help drivers get off the road as quickly and safely as possible. #Hwy401 Follow @OPP_COMM_ER for updates. https://t.co/ov4xoYk1nj— OPP East (@OPP_ER) July 12, 2019\n#LACOPP investigating a multi-vehicle crash on #Hwy401 east of @BellevilleON. 1 fatality, multiple injuries. Ebnd lanes closed for prolonged period. Follow posted EDR, NOT your GPS. If traveling east, advise getting off 401 at Belleville and up 37 to 7. #ONHwys @OPP_COMM_ER ^bd pic.twitter.com/SsuFjBXPCB— OPP East (@OPP_ER) July 12, 2019\nThis latest collision comes only moments after Ontario Provincial Police cleared another serious accident on the same highway, that occurred earlier on Friday morning.\nThe first collision, which is reported to have happened around 5 a.m., also closed the eastbound lanes of the busy 401 Highway. The road was clear again by 3:30 p.m., just minutes before the second incident took place.\nDisclaimer: Cover photo used for illustrative purposes only.