Toronto, Peel and North Bay Parry Sound will officially be entering Ontario's framework next week.
The Ontario government announced on Friday that the stay-at-home order will be lifted as of Monday, March 8 at 12:01 a.m.
This means that both Toronto and Peel will be entering the grey zone, while North Bay will be heading into red.
On top of this, seven other public health regions will be moving to new levels of the framework.
Peterborough Public Health, Public Health Sudbury and Districts, and Simcoe-Muskoka District Health Unit will all see themselves in the red zone on Monday.
Haldimand-Norfolk Health Unit and Timiskaming Health Unit will move to the orange zone.
While the yellow zone will additionally include Haliburton, Kawartha, Pine Ridge District Health Unit and Renfrew County and District Health Unit.
As all regions now officially enter the framework, the government is reminding residents that they should still be avoiding social gatherings and stay home when possible.
"The best defense against the virus and all of its variants of concern remains continuing to stay at home, avoiding social gatherings, only travelling outside of your community for essential purposes, and limiting close contacts to your household or those you live with," stated Dr. David Williams, chief medical officer of health.